For the detection of antibodies to Hepatitis C Virus in human serum or plasma (HCV test kit ELISA)

Model Number: Anti-HCV ELISA 01 Brand Name: Egens Key Specifications/Special Features: This Anti-HCV ELISA is a qualitative enzyme immunoassay for the in vitro detection of antibodies to Hepatitis CVirus(HCV) in human serum or plasma. It is intended for screening of blood donors and diagnosing patientsrelated to infection with Hepatitis C Virus.Assay procedures:1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water,mix well.2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negativecontrol, 2 wells as positive control. After dispensing 100L of Sample Diluent , dispense 10L of sample ornegative control or positive control to the respective wells. Gently vibrating the plate. 3. Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlledwater-bath or microplate incubator at 37℃ for 30 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate: Add 100L of conjugate to each well (except the blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 20 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50L of Substrate Solution A and 50L of Substrate Solution B to each well, mix well.
Cover and incubate at 37℃ for 10 minutes.
9. Stop reaction: Add 50L Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should
be selected from 620nm to 690nm